Figure 3
From: Silencing CDK4 radiosensitizes breast cancer cells by promoting apoptosis
Radiosensitivity imparted by downregulated CDK4 is not caused by altering cell cycle progression. Cells stably expressing pLKO.1 or shCDK4 were unirradiated (basal), or irradiated at 2 Gy. Then, cells were collected at different times post-irradiation (0, 6, 12, & 24 hours) stained with 7-AAD and were analyzed using flow cytometry to determine the cell cycle stages; 5000 cells per group were analyzed by the flow cytometer. Flow cytometry and analysis was repeated three times. The columns represent the percentage of cells in the G1, S or G2/M phase and presented as average±standard deviation. The G1/S and G2/M graphs were separated in order to make the G2/M data visible.