Silencing CDK4 radiosensitizes breast cancer cells by promoting apoptosis

Cell Division

Table 5 Comparison of γ-H2AX between control (pLKO.1) and treatment (shCDK4) groups

Cell line	Vector	6 hr			12 hr			24 hr
Cell line	Vector	Mean*	std	P-value†	Mean	std	P-value†	Mean	std	P-value†
MCF10A	pLKO.1	3.964	1.839	0.4621	3.926	3.203	0.1512	1.308	0.357	3.964
MCF10A	shCDK4	2.803	2.216	0.4621	1.583	1.393	0.1512	1.707	0.643	3.964
MDA-MB-468	pLKO.1	2.148	1.176	0.8272	1.831	0.091	0.9269	4.835	5.890	0.1292
MDA-MB-468	shCDK4	1.660	2.104	0.8272	1.626	1.407	0.9269	1.273	0.729	0.1292
MDA-MB-231	pLKO.1	2.518	0.443	0.4119	3.016	1.634	0.6689	1.587	0.562	0.6609
	shCDK4	3.710	2.273		3.630	3.050		2.217	0.173

* Mean (standard deviation) of the fold change to no radiation.
† P-value is for comparing in the mean fold change no radiation between control and treatment groups.

ISSN: 1747-1028