Nipbl is expressed in mouse spermatocytes. (A) Western blotting assays on total protein extracts from whole mouse testis (Tes) and mouse pre-B cell line (B3)  using antibodies against Nipbl. Anti-Lamin B1 was used as a loading control for each blot. (B) Immunohistochemistry on seminiferous tubule cross-sections from wild-type testis with (left) or without (right) the primary antibody KT55. Nuclei were counterstained with hematoxylin. G, spermatogonium; C, spermatocyte; R, round spermatid; Z, spermatozoon; S, Sertoli cell. Scale bar 200 μm.