Localization of FhkA and CBP4a. (A) Unlike for cells probed with preimmune serum, which displayed diffuse/weak fluorescence, anti-FhkA detected FhkA in untreated cells at the edge of nucleolar patches as demonstrated by colocalization with DAPI counterstain (B) Similarly, cells probed with anti-CBP4a preimmune serum also displayed diffuse/weak fluorescence however anti-CBP4a detected CBP4a in nucleolar patches as demonstrated by colocalization with DAPI counterstain. Merged image displays overlay of FhkA (red), α-tubulin (green), and DAPI (blue). (C) Enlarged view of nuclei showing nucleolar localization pattern of FhkA and CBP4a. Nucleoli are indicated (arrows). At least four independent replicates were performed, all of which showed the same result. Scale bar represents 2 μm.