Nucleolar protrusions are not dependent on microtubules. (A) Nucleus of cell treated with AM-D for 4 hours. (B) Nucleus of cell treated with both AM-D and nocodazole for 4 hours. (C) Enlarged view of protrusion from (B). A merged image shows the overlay of FhkA (red), α-tubulin (green), and DAPI (blue). Scale bar represents 2 μm. At least four independent replicates were performed, all of which showed the same result.