FhkA redistributes throughout the cytoplasm during mitosis. The pattern of microtubules (α-tubulin) was used to determine the stage of mitosis and DAPI was used to identify the nucleus. During prometaphase through telophase FhkA was detected throughout the entire cell. It was also detected at the nuclear envelope region (N). A merged image for each stage shows the overlay of FhkA (red), α-tubulin (green), and DAPI (blue). At least four independent replicates were performed, all of which showed the same result. Scale bar represents 2 μm.