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Figure 6 | Cell Division

Figure 6

From: Rad53 homologue forkhead-associated kinase A (FhkA) and Ca2+-binding protein 4a (CBP4a) are nucleolar proteins that differentially redistribute during mitosis in Dictyostelium

Figure 6

CBP4a redistributes to intranuclear “CBP4a islands” during mitosis. The pattern of microtubules (α-tubulin) was used to determine the stage of mitosis and DAPI was used to identify the nucleus. CBP4a localized to discrete “CBP4a islands” (C) throughout the nucleoplasm during prometaphase through telophase, at least two larger “CBP4a islands” at the metaphase plate region (M) during metaphase, and large “CBP4a islands” around the inner nuclear membrane during telophase. A merged image for each stage shows the overlay of CBP4a (red), α-tubulin (green), and DAPI (blue). At least four independent replicates were performed, all of which showed the same result. Scale bar represents 2 μm.

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