Protein intensity estimation on different background intensities. In each of the 6 images the intensities of the cells and proteins remain the same. Note that each cell contains two bright dots at its poles, which represent the SPBs. However, the image background and the intensity of the simulated petri dish (disk containing the cells) vary from left to right. The bottom row shows the images of the top row with additive random Gaussian white noise (std = 15).