Figure 1From: Cell cycle-dependent localization of CHK2 at centrosomes during mitosis Centrosomes stain positive for CHK2 in human colon cancer cells deficient in CHK2. (A) There is no expression of CHK2 in HCT116 CHK2−/− cells. HCT116 WT and HCT116 CHK2 knockout cells were subjected to Western blotting with anti-CHK2 antibody. (B) We did not detect differences in the staining between WT and CHK2 knockout cells. Exponentially growing HCT116 WT and CHK2−/− cells were fixed and immunostained with antibodies against the N-terminal portion of CHK2 (H-300) or the Thr68-phosphorylated form (P-Thr 68) (green). Cells were costained for γ-tubulin (red) and counterstained with DAPI (blue).Back to article page