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Table 2 Oligonucleotides used in this study with their accompanying mutation identified

From: Mutually dependent degradation of Ama1p and Cdc20p terminates APC/C ubiquitin ligase activity at the completion of meiotic development in yeast

Name

Gene target

Mutation

Created

Oligonucleotide

 

Db1

AMA1

RXXL-AXXA

ATTGTTGGTACAAAATTTGGCGCTATTCTTGCATATGATCAAAAAGAATTTTTTCATTCC

Db2

AMA1

RXXL-AXXA

TTCCCCATAAAAAACTGGAGTAAAGCACGTAAGGCCGAAGATGAAAATTTAATAGGATTGAAA

GXEN

AMA1

GXEN-GXAN

AAATTTTATGTTGGAGAGGCAAATGGCAATGTGAGCCTCTTTGAA