Mutually dependent degradation of Ama1p and Cdc20p terminates APC/C ubiquitin ligase activity at the completion of meiotic development in yeast

Table 3 Plasmids used in this study

Mutation	Gene	Epitope tag	Plasmid name	Promotor	Type	References
WT	AMA1	1 T7	pKC3036	AMA1	2 μ	[12]
CB	AMA1	1 T7	pKC3045	AMA1	2 μ	[12]
IR	AMA1	1 T7	pKC3046	AMA1	2 μ	[12]
CB/IR	AMA1	1 T7	pKC3048	AMA1	2 μ	[12]
Db1	AMA1	1 T7	pKC3126	AMA1	2 μ	This study
GxEN	AMA1	1 T7	pKC3123	AMA1	2 μ	This study
Db1/GXEN	AMA1	1 T7	pKC3127	AMA1	2 μ	This study
Db1/Db2/GXEN	AMA1	1 T7	pKC3129	AMA1	2 μ	This study
3HA	AMA1	3HA	pKC2057	own	Int	[10]
18Myc	CDC20	18Myc	pCdc20-myc18	own	Int	[65]
Codons 1-200	AMA1	GST	pKC3113	GAL	CEN	This study
Codons 1-200 CB	AMA1	GST	pKC3017	GAL	CEN	This study
9HA	Clb1	3HA	pKC427	own	CEN	[32]
3HA	Clb5	3HA	pKC440	own		This study
deletion	Cdh1	No tag	pWS176	own	Int.	[66]
Cb∆/IR∆	AMA1	no tag	pKC3095	T7	-	This study
Cb∆/IR∆/GXEN	AMA1	no tag	pKC3122	T7	-	This study
Cb∆/IR∆/GXEN/Db1	AMA1	no tag	pKC3124	T7	-	This study
Cb∆/IR∆/GXEN/Db1/Db2	AMA1	no tag	pKC3148	T7	-	This study
9Myc	Cdc27	9Myc	Cdc27-9Myc	own	int	P. Hieter
WT	CDC20	no Tag	pME41	T7	-	David Morgan
WT	UBC4	6HIS	6His-Ubc4	T7	-	Mark Solomon
-	GST	-	pEGKT	GAL1 *	2 μ	[67]
1-200CB	GST-AMA1	none	pKC3113	GAL1*	2 μ	[12]
1-200CB	GST-AMA1	none	pKC3017	GAL1*	2 μ	This study

ISSN: 1747-1028