Shugoshin 1 and TTK maintain centrosome amplification in Her2+ breast cancer cells. (A) Basal levels of SGOL1 and TTK are higher in unsynchronized populations of Her2+ breast cancer cell lines compared to MCF10A cells. JIMT-1 cells were transfected with siRNA duplexes against SGOL1 and HCC1954 cells were transfected with siRNA against SGOL1 or TTK. (B) Western blots indicating the transient knockdown of SGOL1 in JIMT-1 and HCC1954 cells (left) and TTK in HCC1954 cells (right). Quantifications of fluorescent microscopy for centrosome amplification by pericentrin staining (C) and of BrdU incorporation (D).