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Table 1 Primers sequences used for real-time PCR and siRNA duplexes

From: Differential expression of centrosome regulators in Her2+ breast cancer cells versus non-tumorigenic MCF10A cells

Primers used for real-time PCR  
Genes Primer sequences
AURAK_F 5′-ATA TCT CAg Tgg Cgg ACg Ag-3′
AURAK_R 5′-TCA AAT ATC CCC gCA CTC Tgg-3′
CDC14B_F 5′-CTC CAT gAA gCg gAA AAg Cg-3′
CDC14B_F 5′-gCA AAA CAA Agg CgA TCg gT-3′
CDK1_F 5′-AAA CTg gCT gAT TTT ggC CT-3′
CDK1_R 5′-ggA gTg CCC AAA gCT CTg AA-3′
CEP192_F 5′-CCC gAg CAC TTg ATT CTg gT-3′
CEP192_R 5′-CCA CTC CAC ggg AAC ATT gA-3′
CETN2_F 5′-AgC ggA CTC CTT Tgg CTA Tg-3′
CETN2_R 5′-gCT CAg gCT TAg ggC TCA TT-3′
GINS2_F 5′-gCC gAg AAg gAg CTg gTT AC-3′
GINS2_R 5′-AAC CAg ggT TAA AAg gCC CC-3′
ROCK2_F 5′-CCC ATC AAC gTg gAg AgC TT-3′
ROCK2_R 5′-TgC CTT gTg ACg AAC CAA CT-3′
SASS6-F 5′-TAC ggA ATg AAT ggg CgT CA-3′
SASS6_R 5′-CTg TgC CTg CAA ggC TTT TT-3′
SPICE_F 5′-ggT CCC CgA gTT ggT gTA Ag-3′
SPICE_R 5′-gCg TAC CAg ATC TTC ggg Ag-3′
SGOL1_F 5′-Agg CAA AAg ATg gCC AAg gA-3′
SGOL1_R 5′-AAA gAC CTg CgT TTg CCA AT-3′
CDK14 F 5′-AAT gAg gAC ACA Tgg CCT gg-3′
CDK14_R 5′-CTg TgC CgA CAg TCT gTT CT-3′
c-Nap_F 5′-AAC CAg CTC Cgg gAg AAA Tg-3′
c-Nap_R 5′-TCT ggC ATA ggg CAC TCT CT-3′
MDM2_F 5′-CCA TgC CTg CCC ACT TTA gA-3′
MDM2_R 5′-CAg gCT gCC ATg TgA CCT AA-3′
PlexinA2_F 5′-ATT TTT CAg CCg AgA ggg Cg-3′
PlexinA2_R 5′-TTT TTC CAg CgC gAC TTT CC-3′
SEMA6A_F 5′-TgA TgC CAA ACA TgC CAA Cg-3′
SEMA6A_R 5′-gCg TCA ATg gCA Agg AAg TC-3′
SFRP1_F 5′-CTC AAC AAg AAC TgC CAC gC-3′
SFRP1_R 5′-CTC gTT gTC ACA ggg Agg AC-3′
Actin_F 5′-CgA ggC CCA gAg CAA gAg-3′
Actin_R 5′-CgT CCC AgT Tgg TAA CAA TgC-3′
TTK_F 5′-CgC AgC TTT CTg TAg AAA TggA-3′
TTK_R 5′-gAg CAT CACTTAGCGGAACAC-3′
siRNA duplexes  
MDM2_1 Sense 5′-rGrGrA rCrCrU rUrGrU rArCrA rArGrA rGrCrU rUrCrA rGrGA A-3′
MDM2_1 Anti-Sense 5′-rUrUrC rCrUrG rArArG rCrUrC rUrUrG rUrArC rArArG rGrUrC rCrUrU-3′
MDM2_2 Sense 5′-rCrCrA rArGrA rCrArA rArGrA rArGrA rGrArG rUrGrU rGrGA A-3′
MDM2_2 Anti-Sense 5′-rUrUrC rCrArC rArCrU rCrUrC rUrUrC rUrUrU rGrUrC rUrUrG rGrGrU-3′
SFRP1_2 Sense 5′-rGrArA rGrCrA rArCrA rGrCrU rUrCrA rGrArA rArGrA rGrCT C-3′
SFRP1_2 Anti-Sense 5′-rGrArG rCrUrC rUrUrU rCrUrG rArArG rCrUrG rUrUrG rCrUrU rCrCrU-3′
SFRP1_3 Sense 5′-rGrArA rArCrA rUrUrU rCrCrU rUrUrG rArArC rUrUrG rArUT G-3′
SFRP1_3 Anti-Sense 5′-rCrArA rUrCrA rArGrU rUrCrA rArArG rGrArA rArUrG rUrUrU rCrUrU-3′
TTK_1 Sense 5′-rGrGrArGrGrUrUrCrArArGrCrArArGrGrUrArUrUrUrCrAGG-3′
TTK_1 Anti-Sense 5′rCrCrUrGrArArArUrArCrCrUrUrGrCrUrUrGrArArCrCrUrCrCrArC-3′
TTK_2 Sense 5′-rCrCrArGrArArUrCrCrUrGrCrUrGrCrArUrCrUrUrCrArAAT-3′
TTK_2 Anti-Sense 5′-rArUrUrUrGrArArGrArUrGrCrArGrCrArGrGrArUrUrCrUrGrGrUrU-3′
SGOL1_1 Sense 5′-rGrArArArUrArUrGrUrUrCrCrUrCrUrGrGrArArUrGrGrACC-3′
SGOL1_1 Anti-Sense 5′-rGrGrUrCrCrArUrUrCrCrArGrArGrGrArArCrArUrArUrUrUrCrCrU-3′
SGOL1_2 Sense 5′-rGrGrArCrUrArCrArGrGrCrArUrGrUrGrCrCrArCrUrArCGC-3′
SGOL1_2 Anti-Sense 5′-rGrCrGrUrArGrUrGrGrCrArCrArUrGrCrCrUrGrUrArGrUrCrCrCrA-3′