Table 2 PCR primers used in this study
From: The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence
Primer | Aplication | Sequence |
|---|---|---|
scm3 F | Gene disruption | TACTGGATTAAAATGCCATGAAAACGTGAACAGAAACTTTTATTGAGGTCCACTCAACCCTATCTCGG |
csm3 R | Gene disruption | TATAGATGCCCACACGCACGTTTGGATTATTACCTTCAATGACATTGCTACTCGAAATTAACCCTCAC |
mrc1 F | Gene disruption | CTAAGGAAGTTCGTTATTCGCTTTTGAACTTATCACCAAATATTTTAGTGCACTCAACCCTATCTCGG |
mrc1 R | Gene disruption | GACAGCTTCTGGAGTTCAATCAACTTCTTCGGAAAAGATAAAAAACCACTCTCGAAATTAACCCTCAC |
tof1 F | Gene disruption | CATCTAGCTTGTGGGGTTTAGTGTATCTTTAATATAGGAGGGCGCACACTCACTCAACCCTATCTCGG |
tof1 R | Gene disruption | TTCTAAAATTACACGTATTAAAGGGATTAATTACTACATATTCATTCTCACTCGAAATTAACCCTCAC |
KanMX-check | Diagnostic PCR | GTCACCTAAATCGTATGT |
csmΔ-check | Diagnostic PCR | ATCGTTTGACAAGAGAGT |
mrc1Δ-check | Diagnostic PCR | TCAAATGTCCAAGTGAAC |
tof1Δ-check | Diagnostic PCR | GAAGAAGTTACTCCAAGA |