Figure 4

Epigenetic changes and protein dynamics at satellite-2 and satellite-α regions during interphase and mitosis in HCT116 and WI-38 cells. qRT-PCR analysis of the satellite-2 (A-C) and satellite-α (D-F) repeats was performed on DNA obtained from anti-H3K4me2, H3K9me2, H3K9me3, H3K9ac, HP1α, HP1β, Mis12 and CENP-A ChIP assays in interphase (black) and mitotic (gray) cells. Normal rabbit IgG was employed as a negative control. ★p < 0.05 and ★★p < 0.01 represent significant differences between interphase and mitosis, as evaluated by Student’s T-test.