Figure 6

Epigenetic changes and protein dynamics at satellite-2 and satellite-α regions after treatment with TSA for 24 and 48 h in HCT116 and WI-38 cells. qRT-PCR analysis of the satellite-2 (A-C) and satellite-α (D-F) repeats was performed on DNA obtained from anti-H3K4me2, H3K9me3, H3K9ac, HP1α, HP1β, Mis12 and CENP-A ChIP assays in untreated cells (Black bar) and TSA-treated cells for 24 (white bar) or 48 h (gray bar). Normal rabbit IgG was employed as a negative control. ★p < 0.05 and ★★p < 0.01 indicate significant differences between treated and untreated cells, as evaluated by Student’s t-test.