Fig. 6From: COP9-Signalosome deneddylase activity is enhanced by simultaneous neddylation: insights into the regulation of an enzymatic protein complexLinear elevation of the Cul1-neddylation fraction promotes linear elevation in p27-ubiquitin conjugated fraction. His-6-p27 was conjugated to methylated ubiquitin for 60 min at 30°C as described above, and with elevated concentrations of UBC12. Blotting was directed against p27 (a) or Cul1 (b).Back to article page