Fig. 1

Imaging of microtubules and mitochondria in dividing HeLa cells. A Spinning disk confocal images of HeLa cells transfected with GFP-Tubulin and stained with MitoTracker Deep Red FM to visualize microtubules (shown in green) and mitochondria (shown in magenta) respectively. Images are maximum projections of five z-slices from the centre of the confocal stack at six representative time-points of division from metaphase to late cytokinesis. Green arrowheads indicate equatorial astral microtubules. (B, C) HeLa cells were stained for mitochondria (MitoTracker Deep Red FM) then fixed and stained for microtubules (anti-α tubulin, shown in green) and visualized by SIM. In (B) images are maximum projections of the full confocal stack. The yellow insets indicate the regions at the cell pole (a), side (b) and equator (c) that have been magnified in adjacent panels. Blue arrowheads indicate mitochondria associated with astral microtubules. The green arrow indicates the direction of equatorial astral microtubules curving towards the cleavage furrow and the blue arrow indicates mitochondria accumulated in a microtubule-devoid region at the equator. The yellow outline indicates the position of the cell cortex. In (C) images are central z slices from the confocal stack of cells in metaphase (top row), early cytokinesis (middle row) and late cytokinesis (bottom row). The yellow insets indicate regions at the cell pole (a) and equator (b) that have been magnified in adjacent panels. Blue arrowheads indicate mitochondria that are associated with microtubules in early and late cytokinesis