Fig. 3
From: Insights into APC/C: from cellular function to diseases and therapeutics
Regulation of APC/C activity. The APC/C activity is governed by catalytic coactivators Cdh1/Cdc20, mitotic checkpoint complex (MCC) and Emi1/2. The MCC complex including MAD2/MAD3, BUB3, BubR1 and Cdc20 generates an inhibitor of APC/C activity to prevent premature anaphase onset. The MCC is able to inhibit both unliganded Cdc20 and Cdc20 bound to the APC/C. Emi1 functions as an APC/C “pseudo-substrate” to block APC/C’s access to other substrates, whereas Emi2 inhibits APC/C by targeting its association with the coactivator Cdc20. The posttranslational modifications, including phosphorylation, could modulate APC/C activity. The large tumor suppressor kinase 1 and 2 (LATS1/LATS2) phosphorylate APC12/CDC26 to modulate TPR lobe assembly and APC/C activity