Skip to main content

Advertisement

Fig. 1 | Cell Division

Fig. 1

From: Interferon gamma-induced apoptosis of head and neck squamous cell carcinoma is connected to indoleamine-2,3-dioxygenase via mitochondrial and ER stress-associated pathways

Fig. 1

a Time course experiment demonstrates cell growth inhibition of HNSCC cell lines CLS-354 and RPMI 2650. Relative cell number (%) assessed by MTT assay following the exposure of cells to IFNγ (1000 U/ml) for regulated time intervals up to 72 h. The values are expressed as the mean ± SD of three independent experiments performed in duplicate. The Student’s t test was used for analysis. b Flow cytometry analysis of the induced apoptosis of CLS-354 and RPMI 2650 cells using annexin V/PI staining following the exposure to IFNγ for 48 h. c Flow cytometry analysis of the mitochondrial membrane potential (Δψm) in CLS-354 and RPMI 2650 cells using JC-1 staining following the exposure to IFNγ for 48 h. d Assessment of ROS level in CLS-354 and RPMI 2650 cells following the exposure to IFNγ for 48 h. ROS generation was measured by flow cytometry using dihydrorhodamine (DHR 123). e Western blot analysis of the pro-apoptotic protein Noxa, cytochrome c, cleavage of caspase-9, caspase-3 and PARP following the exposure of CLS-354 and RPMI 2650 cells to IFNγ for 48 h. Actin was used as internal control for loading and transfer. Data are representative of three dependent experiments performed separately

Back to article page