Fig. 5

a Western blot analysis demonstrates the inhibition of IFNγ-induced phosphorylation of ASK1, JNK and p38 as well as the IFNγ-induced phosphorylation and degradation of IκBα in response to the pre-treatment of CLS-354 and RPMI cells with the scavenger of ROS. Actin was used as internal control for loading and transfer. EMSA analysis demonstrates the inhibition of IFNγ-induced DNA-binding activity of the transcription factors AP-1 (b) and p53 (c) in response to the pre-treatment of CLS-354 cells by the inhibitor of JNK pathway (SP60015). Whereas, the pre-treatment of the same cells with the inhibitors of p38 (SB203580) or the inhibitors of NF-κB pathway (Bay11-7082) was found to block IFNγ-induced DNA-binding activity of ATF-2 (d) and NF-κB (e), respectively. Data are representative of three independent experiments performed separately