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Fig. 5 | Cell Division

Fig. 5

From: Rpl22 is required for IME1 mRNA translation and meiotic induction in S. cerevisiae

Fig. 5

Rpl22 supports translation through the IME1 5′UTR. a Western blot analysis of protein samples prepared from wild type (RSY1833, RSY1991) and rpl22Δ double mutant (RSY1839, RSY1993) strains harboring IME1-3HA or 5′UTRΔ-IME1 alleles, respectively. The blots were probed for Ime1-3HA and Pgk1, which served as a loading control. b The Ime1 signals obtained in a were quantified, normalized to Pgk1 levels, and plotted for each timepoint (see “Methods” section for details). c IME2 mRNA levels were determined by qRT-PCR during a meiotic timecourse with the strains described in a. The results depicted are the average from three technical replicates from one experiment. d Terminal sporulation efficiencies following 24 h in SPM were determined for the strains described in a. The values for rpl22Δ and 5′UTRΔ-IME1 are the result of two trials of three independent cultures. Error bars indicate SEM. Asterisk indicates p = 0.05

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