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Fig. 2 | Cell Division

Fig. 2

From: The Irr1/Scc3 protein implicated in chromosome segregation in Saccharomyces cerevisiae has a dual nuclear-cytoplasmic localization

Fig. 2

Irr1 appears in the cytoplasm in the G1 phase of the cell cycle. a Yeast culture was subjected to α-factor treatment as in Fig. 1, then allowed to resume the cell cycle by washing out the pheromone (arrow) and incubating the cells in YPD medium. At times indicated aliquots were withdrawn, fixed in formaldehyde and inspected for Irr1-GFP localization. Spc42-mCherry, DAPI and DIC as in Fig. 1. Cell-cycle phases inferred from cell and nuclear morphology are indicated on the right. b GFP fluorescence distribution between nucleus and cytoplasm. Signal intensity ratio was determined as described in “Methods” section and is shown as mean ± SD for 25 cells per experimental variant

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