Table 1 Saccharomyces cerevisiae strains

IRR1

MAT a ade2—1 his3-11,15 leu2-3112 trp1-1 ura3-1 can1-100

Rothstein collection (Columbia University, New York, USA)

2281^a

MAT a/α {ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 irr1::IRR1-GFP/kanMX4 spc42::SPC42-mCherry/natMX6}

D. Ivanov, Inst. Mol. Cell Biology, Singapore [25]

2239^a

MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 irr1::IRR1-GFP/kanMX4 spc42::SPC42-mCherry/natMX6

D. Ivanov

PJ69-4α

MAT α trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ

[33]

Irr1-GFP/Imi1-RFP

MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 irr1::IRR1-GFP/kanMX4

[pCM189-IMI1-RFP, URA3, CEN]

This study, derivative of 2239

Irr1-GFP/RFP

MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 irr1::IRR1-GFP/kanMX4

[pCM189-RFP, URA3, CEN]

This study, derivative of 2239

GFP/Imi1-RFP

MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 imi1::natMX6 [pUG34-GFP, HIS3, CEN] [pCM189-IMI1-RFP, URA3, CEN]

This study, derivative of 2239

MNY7

MAT a trp1 his3 leu2 ura3 crm1::kanR [pDC-CRM1, LEU2, CEN]

[34]

MNY8

MAT a trp1 his3 leu2 ura3 crm1::kanR [pDC-crm1-T539C, LEU2, CEN]

[34]

MNY7-IRR1-GFP

MAT a trp1 his3 leu2 ura3 crm1::kanR IRR1::irr1-GFP/kanMX4 spc42::SPC42-mCherry/natMX6

[pDC-CRM1, LEU2, CEN]

This study, derivative of MNY7

MNY8-IRR1-GFP

MAT a trp1 his3 leu2 ura3 crm1::kanR IRR1::irr1-GFP/kanMX4 spc42::SPC42-mCherry/natMX6

[pDC- crm1-T539C, LEU2, CEN]

This study, derivative of MNY8

irr1(V248E)-GFP

MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 irr1:: kanMX4

[pUG35-irr1(V248E)-GFP, URA3, CEN]

This study, derivative of IRR1

irr1(F986A)-GFP

MAT a ade2-1 his3-11,15 leu2-3112 trp1-1 ura3-1 can1-100 irr1:: kanMX4

[pUG35-irr1(F986A)-GFP, URA3, CEN]

This study, derivative of IRR1

Irr1(V248E, F986A)-GFP

MAT a ade2-1 his3-11,15 leu2-3112 trp1-1 ura3-1 can1-100 irr1:: kanMX4

[pUG35-irr1(V248E, F986A)-GFP, URA3, CEN]

This study, derivative of IRR1