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Table 2 Plasmids used in this study

From: The Irr1/Scc3 protein implicated in chromosome segregation in Saccharomyces cerevisiae has a dual nuclear-cytoplasmic localization

Plasmid Description Source:
pACTII P ADH1 -Gal4-AD-MCS- T ADH1 Amp R LEU2 Clontech #638822
pGBKT7 P ADH1 -Gal4-DNA-BD-MCS-T T7 & ADH1 Kan R TRP1 2 μ Clontech #630489
pGBKT7-C-Irr1 P ADH1 -Gal4-DNA-BD-Irr1Δ1-474-T T7 & ADH1 Kan R TRP1 This study
pCM189 P tetO -MCS-T CYC1 Amp R URA3 CEN [37]
pCM189-P tetO -IMI1-RFP P tetO -IMI1-RFP-T CYC1 Amp R URA3 CEN [30]
pCM189-P tetO -RFP P tetO -RFP-T CYC1 Amp R URA3 CEN This study
pUG23 P MET25 -MCS-GFP-T CYC1 Amp R HIS3 CEN U. Güldener and J. H. Hegemann, Heinrich-Heine-Universität, Düsseldorf, Germany
pUG34 P MET25 -GFP-MCS-T CYC1 Amp R HIS3 CEN