Fig. 2

Effect of Eg5 inhibition on the spindle assembly and chromosome alignment in porcine oocytes. a Representative images of spindle morphologies and chromosome alignment in control and Eg5-inhibited oocytes. Oocytes were immnunostained with anti-α-tubulin-FITC antibody to visualize the spindles and counterstained with Hoechst to visualize the chromosomes. Scale bar, 5 μm. b The rate of aberrant spindles was recorded in control and Eg5-inhibited oocytes. c The rate of misaligned chromosomes was recorded in control and Eg5-inhibited oocytes. Data in b, c were presented as mean percentage (mean ± SEM) of at least three independent experiments. **p < 0.01