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Fig. 3 | Cell Division

Fig. 3

From: Eg5 orchestrates porcine oocyte maturational progression by maintaining meiotic organelle arrangement

Fig. 3

Effect of Eg5 inhibition on the acetylation level of α-tubulin in porcine oocytes. a Representative images of acetylated α-tubulin in control and Eg5-inhibited oocytes. Oocytes were immnunostained with anti-acetyl-α-tubulin (Lys-40) antibody to assess the acetylation level of α-tubulin. Scale bar, 5 μm. b Quantitative analysis of the fluorescence intensity of acetylated α-tubulin in control and Eg5-inhibited oocytes. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. **p < 0.01. c The acetylation levels of α-tubulin in control and Eg5-inhibited oocytes were examined by western blotting. The blots were probed with anti-acetyl-α-tubulin (Lys-40) antibody and anti-Gapdh antibody, respectively

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