Fig. 2

IMP promotes IMPDH cytoophidium formation. a A simplified diagram showing IMP-related metabolism. b Immunofluorescence for IMPDH2 in HEK 293T cells treated with DMSO or allopurinol (50 μM) for 1 h. c Mean (± SEM) percentages of HEK 293T cells with IMPDH cytoophidia after various doses of allopurinol treatment for 1 h. (Student’s t-test, *P < 0.05, ***P < 0.001). d Immunofluorescence of IMPDH2 antibody labelled IMPDH cytoophidia in OFP-P2A-GMPR-expressing HeLa cells. Cells with high fluorescence intensity indicated by arrows and cells with low intensity are indicated by arrowheads. e Average (± SEM) number of cytoophidia per cell in cells with high versus low OFP fluorescence intensity expressing OFP-P2A-GMPR_wt. f Average (± SD) size of cytoophidium in cells with high versus low OFP fluorescence intensity. g Immunofluorescence of HeLa cells expressing catalytic dead OFP-P2A-GMPR constructs. h Mean (± SD) of HeLa cells with IMPDH cytoophidia after transfection with different GMPR plasmids (Student’s t-test, ***P < 0.001). Nuclei were labelled with DAPI (blue) in all images and scale bar = 20 μm in b and 10 μm in d and g