Fig. 6

Knockdown of IMPDH2 in IMPDH1-KO cells impairs cell proliferation. a Sequences showing the Cas9 cutting site and indels in IMPDH1 gene of IMPDH1-KO cells. b Immunofluorescence of IMPDH1-KO cell line after treatment with 1 mM ribavirin for 4 h. c IMPDH1-KO cells were transfected with NT siRNA or 0.1 or 100 nM of IMPDH2 siRNA, and labelled with EdU and anti-IMPDH2 antibody. Proportion of cells labelled by EdU is presented in the top of each panel and proportion of cells containing cytoophidia is presented below each panel. More than 700 cells were counted for each group. d Proliferation rate of each IMPDH1-KO group normalized with the group transfected with Non-Target siRNA. e Quantitative data of cells labelled by EdU in c. f IMPDH2 protein levels in WT and IMPDH1-KO cells indicating a decrease in protein levels with various doses of siRNA for IMPDH2 knockdown. No IMPDH1 was found in IMPDH1-KO cell line. For statistics, groups were compared by Student’s t-test as indicated, **P ≤ 0.01, ***P ≤ 0.001