Fig. 5

PGRMC1 localizes to cold-stable kinetochore fibers. Cells were cold-treated and stained with anti-PGRMC1 (green), anti-α-tubulin (TUBA) (red), anti-CENPB (red), anti-NDC80 (red), anti-TACC3 (red) and DAPI (blue). a Human adrenocortical NCI-H295R cells. b Human skin fibroblasts of healthy wild-type donors. c Human skin fibroblasts of triple A patients. The different mutations in the human ALADIN protein are denoted as IVS14, S263P and W295X. Scale bars 5 µm