Fig. 2

Inhibition of cell growth and mitotic division after exposure to 3,5,4′-TMS or 3,4,4′-TMS. Growth curves of HCT116 cells treated with different concentrations of 3,5,4′-TMS (a) or 3,4,4′-TMS (b). Time course analysis of cell cycle distribution by flow cytometry following 3,5,4′-TMS (c) or 3,4,4′-TMS (d) exposure. Frequency of mitoses following 3,5,4′-TMS (e) or 3,4,4′-TMS (f) exposure. g Western blot analysis of ser10 phosphorylated histone H3 (pH3(ser10)) after 15 or 24 h exposure to 3,5,4′-TMS or 3,4,4′-TMS. GAPDH is used as loading control. h Frequency of prometa/metaphase cells (Pro/Meta) and anaphase/telophase cells (Ana/Telo) in HCT116 mitoses collected after 15 h treatment with 3,4,4′-TMS. Values are the mean ± SE of 3–4 independent experiments. *: p < 0.05; **: p < 0.01; ***: p < 0.001 compared with controls