Fig. 3

Induction of apoptotic cell death following exposure to 3,5,4′-TMS or 3,4,4′-TMS. Representative flow cytometric histograms of HCT116 cells at 48 h following 3,5,4′-TMS (a) or 3,4,4′-TMS (b) treatment. X axis = DNA content, Y axis = number of events. Quantitative analysis of the percentage of hypodiploid (< 2C) and polyploid (> 4C) cells after 24 or 48 h exposure to 3,5,4′-TMS (c) or 3,4,4′-TMS (d). Data are mean ± SE of 2–4 independent experiments. *: p < 0.05; **: p < 0.01; ***: p < 0.001 compared with controls. Flow cytometric analysis of Annexin V-FITC reactivity of HCT116 cells treated for 48 h with 20 μM of 3,5,4′-TMS or 3,4,4′-TMS (e)