Fig. 4

Alterations of mitotic spindle structure upon treatment with 3,5,4′-TMS or 3,4,4′-TMS. a HCT116 cells were treated with 20 or 80 µM 3,5,4′-TMS or 3,4,4′-TMS for 2 h, fixed and stained with anti-α-tubulin and anti-γ-tubulin antibodies. 3D projections of confocal images of untreated and treated cells are shown. Scale bar, 10 μm. b Quantitative analysis of MT and centrosome alterations in control and treated cells. Values are the mean ± SE of 2 independent experiments. *: p < 0.05; **: p < 0.01; ***: p < 0.001