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Fig. 5 | Cell Division

Fig. 5

From: Overexpression of P16 reversed the MDR1-mediated DDP resistance in the cervical adenocarcinoma by activating the ERK1/2 signaling pathway

Fig. 5

Analysis of the expression of ERK1/2, pERK1/2, and P-gp by Western blot. ac The expression of pERK1/2 and P-gp in HeLa/DDP cells after transfection with pEX-2 empty vector or P16 plasmid. a Representative images of ERK1/2, pERK1/2, and P-gp. b Quantitative analysis of pERK1/2 protein expression relative to that of total ERK1/2. c Quantitative analysis of P-gp expression relative to that of β-actin. df Effects of U0126 on the expression of pERK1/2 and P-gp in HeLa/DDP cells at different time points. d Representative images of ERK1/2, pERK1/2, and P-gp. e Quantitative analysis of the pERK1/2 protein expression relative to total ERK1/2 expression. f Quantitative analysis of the P-gp expression relative to β-actin expression. gi Effects of TPA on the expression of pERK1/2 and P-gp in HeLa/DDP cells at different time points. g Representative images of ERK1/2, pERK1/2, and P-gp. h Quantitative analysis of the pERK1/2 protein expression relative to total ERK1/2 expression. i Quantitative analysis of the P-gp expression relative to β-actin expression. jl Effects of U0126 on the expression of pERK1/2 and P-gp in HeLa/DDP cells after P16 transfection at different time points. j Representative images of ERK1/2, pERK1/2, and P-gp. k Quantitative analysis of the pERK1/2 protein expression relative to total ERK1/2 expression. l Quantitative analysis of the P-gp expression relative to β-actin expression. A, P < 0.05 versus CON (control) group; B, P < 0.05 versus 24 h group; C, P < 0.05 versus 48 h group

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