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Fig. 3 | Cell Division

Fig. 3

From: M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s

Fig. 3

The comparison of the amount of slippage and mitotic status of the HeLa cells caused by the various drug treatments. The cells used are the same cells as for Fig. 2. a An example of confocal time-lapse images showing a HeLa H2B-GFP cell undergoing slippage. The white arrows indicate the chromosomes that are undergoing premature decondensation without cytokinesis having taken place. The white dash line arrows highlight the re-attachment of the cytoplasmic membrane. b The quantitative cell death that occurred after prolonged mitotic arrest from cells treated with 60 ng/ml nocodazole alone (grey border) or 60 ng/ml nocodazole + 50 μM M2I-1 treatment (black border). The results were produced from three independent experiments. *P value: < 0.012, **P value < 0.002. c Quantitative results showing the mitotic indices due to the different drug treatments (color-coded as above) at different time intervals. **P value < 0.002. d Quantitative results showing the mitotic indices due to the different drug treatments (black/grey borders the same as above) at different time intervals as indicated. P values shown as indicated. e Quantitative results showing the apoptotic indices due to the different drug treatments (color-coded the same as above) at different time intervals as indicated. P values shown as indicated. f Quantitative results comparing the slippage rates between different drug treatments (color-coded same as above). The data from three independent experiments were used for the quantifications. n, cell numbers used for quantification; ns, not significant

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