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Fig. 6 | Cell Division

Fig. 6

From: M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s

Fig. 6

M2I-1 induced the down-regulation of Cyclin B1. a Representative confocal images which show the selected cell cycle stages of early prophase (Early Pro), prophase (Pro), prometaphase (ProM), and metaphase (Met) of HeLa cells after treatment with 0.5% DMSO (control) and 50 μM M2I-1 respectively. Fixed and rehydrated slides were treated with anti-Cyclin B1 antibodies for a PLA assay, after the PLA they were also probed using a pericentrin antibody and the DNA stained using DAPI. The cyclin B1 signal is in red (white arrowheads), pericentrin in green, and the DNA stained with DAPI in blue. The DNA morphologies and the centrosome status indicated by white arrows (Pericentrin staining) were used for determining the cell cycle stages as indicated. The nuclear and cytoplasmic regions are encircled by the dash lines. b Quantitative results comparing the levels of cyclin B1 at prophase, or prometaphase and metaphase based on single cell analysis from the cell population gathered from a. P value **** < 0.0001

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