Fig. 3
From: Nucleoporin Nup58 localizes to centrosomes and mid-bodies during mitosis
Colocalization of Nup58 with midbody protein markers. a Total cell extracts of interphase cells and extracts of purified midbodies were examined by immunoblotting with the indicated antibodies. b Categorization of midbody proteins according to localization on the midbody. c, d Deconvoluted confocal images of HeLa cells during stages of cytokinesis. e, f Super-resolution images of Nup58 and KIF4 acquired with confocal and STED microscopy, respectively. Green, anti-Nup58 ab; red, anti-γ-tubulin (c) or anti-KIF4 (d–f). Line scans show the positions of proteins in relation to each other