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Fig. 4 | Cell Division

Fig. 4

From: Nucleoporin Nup58 localizes to centrosomes and mid-bodies during mitosis

Fig. 4

Localization of Nup58 on the midbody during cytokinesis. a Cell lysates of HeLa cells transfected with Nup58–GFP expression plasmid or GFP-vector were analyzed by immunoblotting for GFP or Nup58–GFP. b Live-cell images of GFP-vector-expressing HeLa cells during cytokinesis from midbody maturation to final abscission (Additional file 3: Video 2). c Live-cell images of Nup58–GFP-expressing HeLa cells during cytokinesis from midbody maturation to final abscission showing localization of Nup58 on the midbody. Insets are magnifications of midbody areas in observed cells (Additional files 4, 5: Video 3a, 3b). Arrowheads indicate HeLa cell midbodies during cytokinesis. Scale bars, 5 µm

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