Fig. 1
From: Septin and Ras regulate cytokinetic abscission in detached cells
Active Ras, but not SV40 LT protein, promotes the recruitment of ALIX to the MB in detached cells. Mitotic BJ-LT (a, b) and BJ-LT-Ras (c, d) cells were isolated and cultured for the indicated time periods in suspension or on fibronectin. a, c Representative immunofluorescence micrographs illustrating the cells immunostained for Aurora B (green; MB marker), CEP55 (green; MB marker) and ALIX (red). Nuclei were stained with DAPI (blue). b, d Mean% ± SD of the number of cells having ALIX at the MB. After 3 h no midbody is detected because the cells have entered G1; in the adherent cells abscission has been completed at this time point