Fig. 8

Bi-nucleated BJ cells with regressed ICB accumulate nuclear p21. Representative immunoflourescence images illustrating the distribution of septin-7 (green) and p21 (red) in mono- and bi-nucleated BJ and BJ-LT cells [also labelled for DNA (blue)]. a The isolated mitotic BJ cells were either seeded directly on fibronectin for 42 + 18 h (control), cultured in suspension for 42 h, or cultured in suspension for 42 h before re-plating on fibronectin-coated coverslips for 18 h. Alternatively, cells seeded on fibronectin were incubated for 1 h with or without CytD and further incubated for 42 + 18 h without CytD. b Mean% ± SD of BJ cells with positive staining for p21 in the nucleus under the conditions described in a. c Mean% ± SD of the BJ cells with positive staining for nuclear p21 in cell having or lacking septin-7 at the ICB after 42 h in suspension culture. d Mitotic BJ-LT cells seeded on fibronectin were incubated for 1 h with or without CytD and further incubated for 18 h without CytD