Fig. 2

LHX9 knockdown impaired the proliferation of OS cell lines. a–c U2OS cells or Saos-2 cells were transfected with negative control shRNA (shNC) or shRNA targeting LHX9 (shLHX9), then they were cultured for 48 h. a The expression of LHX9 in shNC- or shLHX9-transfected cells was measured by Western blot. GAPDH was used as the loading control. The relative expression of LHX9 after normalization to GAPDH was shown in the right histogram. b The proliferation of shNC- or shLHX9-transfected cells was determined by CCK-8 assay. c The survival and proliferation of shNC- or shLHX9-transfected cells were verified by colony formation assay. The statistics of colony formation was shown in the right histograms. a–c The result was a representative of three independent experiments. a–c Error bars represented mean ± SD. p values were determined by unpaired two-tailed Students’ t-test (a, c) or two-way analysis of variance (ANOVA) with Tukey’s post hoc test (b). **p < 0.01, *p < 0.05