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Fig. 2 | Cell Division

Fig. 2

From: FGF-induced LHX9 regulates the progression and metastasis of osteosarcoma via FRS2/TGF-β/β-catenin pathway

Fig. 2

LHX9 knockdown impaired the proliferation of OS cell lines. ac U2OS cells or Saos-2 cells were transfected with negative control shRNA (shNC) or shRNA targeting LHX9 (shLHX9), then they were cultured for 48 h. a The expression of LHX9 in shNC- or shLHX9-transfected cells was measured by Western blot. GAPDH was used as the loading control. The relative expression of LHX9 after normalization to GAPDH was shown in the right histogram. b The proliferation of shNC- or shLHX9-transfected cells was determined by CCK-8 assay. c The survival and proliferation of shNC- or shLHX9-transfected cells were verified by colony formation assay. The statistics of colony formation was shown in the right histograms. ac The result was a representative of three independent experiments. ac Error bars represented mean ± SD. p values were determined by unpaired two-tailed Students’ t-test (a, c) or two-way analysis of variance (ANOVA) with Tukey’s post hoc test (b). **p < 0.01, *p < 0.05

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