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Fig. 5 | Cell Division

Fig. 5

From: FGF-induced LHX9 regulates the progression and metastasis of osteosarcoma via FRS2/TGF-β/β-catenin pathway

Fig. 5

Beta-catenin knockdown resulted in the attenuation of LHX9 and metastasis-related proteins. U2OS or Saos-2 cells were transfected with shNC or shRNA targeting beta-catenin (shbeta-catenin), then they were cultured for 48 h. The expression of β-catenin, LHX9, COL1A1, MMP-1, Snail-1, Slug-1, Twist-1 and MMP-9 was assessed by Western blot. GAPDH was used as the loading control. The normalized expression of these proteins against GAPDH was shown in the right histograms. The result was a representative of three independent experiments. Error bars represented mean ± SD. p values were determined by unpaired two-tail Students’ t-test. **p < 0.01, *p < 0.05

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