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Fig. 6 | Cell Division

Fig. 6

From: FGF-induced LHX9 regulates the progression and metastasis of osteosarcoma via FRS2/TGF-β/β-catenin pathway

Fig. 6

FRS2 knockdown resulted in the up-regulation of LHX9, BMP4, β-catenin, let-7c and TGF-βR1. a, b U2OS or Saos-2 cells were transfected with shNC or shRNA targeting FRS2 (shFRS2), then they were cultured for 48 h. a The expression of LHX9, BMP4, β-catenin, and TGF-βR1 was assessed by Western blot. GAPDH was used as the loading control. The normalized expression of these proteins against GAPDH was shown in the right histograms. b The expression of let-7c was measured by RT-qPCR. Let-7c were normalized to GAPDH mRNA (n = 3). a, b The result was a representative of three independent experiments. Error bars represented mean ± SD. p values were determined by unpaired two-tail Students’ t-test (a, b). **p < 0.01, *p < 0.05

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