Mechanisms for the temporal regulation of substrate ubiquitination by the anaphase-promoting complex/cyclosome

Cell Division

Table 1 Motifs recognised by CDC20 and CDH1 in known APC/C substrates

Motif	Consesus sequence	Coactivator	Substrate	Function	References
D-box	Rx^axLx[D/E][Ø]xN[N/S]	CDC20, CDH1	Securin, Cyclins	Proteolytic degradation	[70]
KEN-box	KENxxxN	CDH1	CDC20, NEK2A	Proteolytic degradation	[145]
ABBAmotif	Fx[ILV]^c[FHY]x[DE]	CDC20	Cyclin A, BUBR1, BUB1 and ACM1	Proteolytic degradation	[71]
A-box	QRILGPS*^bNVPQRV	CDH1	Aurora A kinase	Regulatory domain for proteolytic degradation	[35]
O-box	AS[P]LT[E][K][N][A]K	CDH1	ORC1	Proteolytic degradation	[73]
CRY-box	CRYxPS	CDH1	CDC20	Proteolytic degradation	[72]
GxEN	GxEN	CDC20, CDH1	XKID (Xenopus chromokinesin Kid)	Proteolytic degradation	[74]
SPO13	QK[P]LQ[E][K][T][P]N	CDH1	SPO13	Proteolytic degradation	[146]
CIN8P	KM[P]LR[L][S][N][I]N	CDH1	CIN8P	Proteolytic degradation	[147]
TEK-box	R/KxxTxKT	CDH1	Securin	K-11 ubiquitination	[148]
C-box	DRYIPHR	CDH1, CDC20	APC	APC/C association	[64]

ISSN: 1747-1028