Fig. 2
From: Kinesin-5 Eg5 is essential for spindle assembly and chromosome alignment of mouse spermatocytes
Eg5 inhibition resulted in the increase of monopolar spindles in mouse spermatocytes and the disruption of seminiferous tubules. a Representative images of the immunofluorescence of the seminiferous tubules in the Control, Monastrol (50 μM), STLC (10 μM) and Dimethylenastron (20 μM) groups. DAPI (blue) and β-tubulin (green). Scale bar, 25 μm. The enlarged images of metaphase-arrested spermatocytes and abnormal spermatogenic cells were shown in the zoom. The misaligned chromosomes and monopolar spindles were shown. Scale bar, 5 μm. b HE staining of the seminiferous tubules in the Control and STLC (10 μM) groups. Representative images of stage I, V, IX and XI were shown. Scale bar, 50 μm. The magnified images of dashed boxes were shown. Scale bar, 20 μm (Zoom)