Fig. 5

The cultured GC-2 spd cells arrested at metaphase during the cell cycle after Eg5 inhibition. a Cell-cycle analyses of the GC-2 spd cells incubated with 1 μM STLC for 24 h (n = 3000). The black box indicated the selected cell population of GC-2 spd cells. The G₀/G₁, G₂/M and S-phase were calculated by the Modfit software and were shown in the peak figures. b The ratios of G₀/G₁ cells in the Control and STLC groups (Control, 52.78 ± 0.52%; STLC, 14.68 ± 0.10%). n = 3 per group. c The ratios of G₂/M cells in the Control and STLC groups (Control, 7.52 ± 0.94%; STLC, 61.44 ± 1.04%). n = 3 per group. d The ratios of S-phase cells in the Control and STLC groups (Control, 39.69 ± 0.54%; STLC, 23.88 ± 1.04%). n = 3 per group. e Giemsa staining of the GC-2 spd cells incubated with 1 μM STLC and 1 μM Dimethylenastron for 14 h. Scale bar, 100 μm. The enlarged images indicated prometaphase cells (a’), dead cells (b’), heteromorphic nuclear cells (c’) and multinucleated cells (d’). Scale bar, 10 μm. f The ratios of cells in prometaphase in the Control, STLC and Dimethylenastron groups (Control, 2.31 ± 0.61%; STLC, 14.31 ± 2.64%; Dimethylenastron, 13.74 ± 1.15%). n = 7, 8, 9. g The ratios of dead cells in the Control, STLC and Dimethylenastron groups (Control, 1.29 ± 0.29%; STLC, 7.08 ± 0.58%; Dimethylenastron, 8.79 ± 0.81%). n = 7, 8, 9. h The ratios of heteromorphic nuclear cells in the Control, STLC and Dimethylenastron groups (Control, 3.53 ± 1.17%; STLC, 9.29 ± 0.97%; Dimethylenastron, 11.78 ± 1.34%). n = 7, 8, 9. i The ratios of multinucleated cells in the Control, STLC and Dimethylenastron groups (Control, 4.90 ± 0.47%; STLC, 8.90 ± 1.07%; Dimethylenastron, 6.29 ± 0.87%). n = 7, 8, 9. Student’s t-test. Error bars, mean ± SEM. ns, p > 0.05; **, p < 0.01; ***, p < 0.001 and ****, p < 0.0001