Fig. 2

EZH2 inhibition repressed mTORC1 and mTORC2 activity. NRK-52E cells were stimulated with 20 μM DZNep for the indicated times (a, c) or stimulated with different concentrations of DZNep for 24 h (b, d). Western blots were used to measure the protein level of p ~ S6K, S6k, p ~ Akt and Akt. GAPDH was used to verify equivalent loading, and quantitative data are presented. Data are given as the mean ± SD of three independent experiments. *p < 0.05 versus vehicle-treated cells