Fig. 1

XMU-MP-1 perturbs cell cycle progression in proliferating human hair matrix keratinocytes. a Treatment of human hair follicles with 3 µM XMU-MP-1 for 24 h significantly decreases the number of Ki-67 + cells in the hair matrix, whilst simultaneously increasing Ki-67 protein expression (p = 0.0002 for both analyses). Mann–Whitney test performed on 7–9 hair follicles from 3 donors. b XMU-MP-1 significantly (p =0.0107) decreases the number EdU + cells (S-phase). Unpaired t-test performed on 10 hair follicles per group from 3 donors. c XMU-MP-1 significantly (p ≤ 0.0001) decreases the number of mitotic phospho histone H3 (pH3) + cells. Mann–Whitney test performed on 7–9 hair follicles from 3 donors. d Immunofluorescence images show that XMU-MP-1 perturbs markers of cell cycle progression in the human hair matrix. 50 µm scale. e XMU-MP-1 does not affect the immunoreactivity of active YAP1 in the proliferative hair matrix but significantly (p =0.028) decreases active YAP1 immunoreactivity in the hair follicle pre-cortex. Mann–Whitney test performed on 7-8 hair follicles from 3 donors. f XMU-MP-1 significantly (p ≤ 0.0001) decreases phospho-MOB1 (Thr35) immunoreactivity in the hair follicle matrix and pre-cortex. Unpaired t-test performed on 9–10 hair follicles from 3 donors. g Immunofluorescence images show that XMU-MP-1 selectively decreases active YAP1 immunoreactivity in the hair follicle pre-cortex and decreases phospho-MOB1 (Thr35) immunoreactivity in the hair follicle matrix and pre-cortex. Error bars represent standard error. FI Fluorescence intensity, HM Hair Matrix