Fig. 3

Establishment of single HDF-derived karyotypically normal clones by KMOS protein transduction. a The schematic protocol depicts a repeated process and the timeline for generating vigorous HDF clones. b Representative image of a HDF clone of control group (left) and a vigorous HDF clone induced by KMOS proteins (right). c Quantification of cloning efficiencies in control groups and KMOS protein treatment groups. d Representative images of EDU⁺ cells in primary HDF cultures (left) and the cells amplified from vigorous HDF clone (right). EDU (Life Technologies, MA) was added to a final concentration of 10 µM for 24 h. e Quantification of EDU⁺ cells in primary HDF cultures and KMOS protein-treating groups. f Representative karyotype of vigorous HDF clone, no chromosomal aberration was detected. Statistical analyses are presented as Mean ± SD, n = 3. *P < 0.05, **P < 0.01. Scale bars, 100 µm