Fig. 2

Inverse regulation of EZH2 and NOX4 with aging. Immunofluorescence images of frozen sections from NP tissues. Nuclei staining (DAPI), NOX4 and EZH2 staining (a) and p16 (b) staining are depicted in blue, green and red (n = 3). Cells in the ellipses were highly expressed NOX4 but lowly expressed EZH2. Cells in the squares were in the high expression of EZH2 and low expression of NOX4. Scale bar, 10 μm. NP paraffin sections stained with NOX4 (c), EZH2 (d), and p16 (Additional file 1: Figure S1) showed significant differences in 2 M and 10 M rats. e Show the mean OD of NOX4, EZH2, and p16 in NP sections. The data are represented as the mean ± SEM (n = 11). f Immunoblot analysis and OD analysis of NOX4, EZH2, and p16 in NP tissues extracted from 2 M rats and 10 M rats. β-actin was used as a loading control. The data are represented as the mean ± SEM (n = 4). **p < 0.01. *p < 0.05